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TECK Thymus Expressed Chemokine Mouse Recombinant Protein (CCL25)

TECK Mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 14.1kDa. The TECK is purified by proprietary chromatographic techniques.

Product Specifications

Synonyms

C-C motif chemokine 25; Small-inducible cytokine A25; Thymus-expressed chemokine; Chemokine TECK; CCL25; SCYA25; TECK; Ckb15; MGC150327

Gene Name

CCL25

UniProt

O35903

Reactivity

Mouse

Tissue Specificity

Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.

Source

Escherichia coli

Sequence

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Purification

Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Bioactivity

Determined by its ability to chemoattract human CCR9 transfected BaF3 mouse pro-B cells using a concentration range of 0.1-0.5 ug/ml.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

It is recommended to reconstitute the lyophilized TECK in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Function

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Shipping Conditions

Available

Storage Conditions

Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Other Gene Names

C-C motif chemokine 25

Subcellular Location

Secreted.

Available Sizes

Frequently Asked Questions

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