Lysostaphin Recombinant Protein
Selected Variant
PROTP10547-01
Size: 1 mg
Lysostaphin Recombinant produced in E. coli is a non-glycosylated polypeptide chain having a molecular mass of 26.92 kDa.
Product Specifications
Synonyms
Lysostaphin; EC 3.4.24.75; Glycyl-glycine endopeptidase
Gene Name
LSS
UniProt
P10547
Source
Escherichia coli
Purification
95% as determined by RP-HPLC.
Bioactivity
Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8, 30°C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor.
Form
Sterile Filtered lyophilized powder.
Reconstitution
It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted to other aqueous solutions such as 200mM Tris HCL pH-8 for optimal activity.
Function
Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
Shipping Conditions
Available
Storage Conditions
Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Other Gene Names
Lysostaphin
Subcellular Location
Secreted.
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