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SOST Sclerostin Human Recombinant Protein

SOST Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain (a.a 24-213) containing 200 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 22.8kDa (calculated) .

Product Specifications

Synonyms

Sclerostin; SOST; CDD; VBCH

Gene Name

SOST

UniProt

Q9BQB4

Reactivity

Human

Tissue Specificity

Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level) .

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 90.0% as determined by SDS-PAGE.

Form

Filtered White lyophilized (freeze-dried) powder.

Reconstitution

Add 0.1M acetate buffer pH4 to prepare ~0.5mg/ml working stock solution. For higher pH, dilute with relevant buffer to 10µg/ml. The antigen has limited solubility in higher concentrations. Non-sterile product; filter before use in cell culture.

Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Shipping Conditions

Available

Storage Conditions

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Other Gene Names

Sclerostin

Subcellular Location

Secreted, extracellular space, extracellular matrix

Available Sizes

Frequently Asked Questions

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