SOST Sclerostin Human Recombinant Protein
SOST Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain (a.a 24-213) containing 200 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 22.8kDa (calculated) .
Product Specifications
Synonyms
Sclerostin; SOST; CDD; VBCH
Gene Name
SOST
UniProt
Q9BQB4
Reactivity
Human
Tissue Specificity
Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level) .
Source
Escherichia coli
Sequence
[object Object]
Purification
Greater than 90.0% as determined by SDS-PAGE.
Form
Filtered White lyophilized (freeze-dried) powder.
Reconstitution
Add 0.1M acetate buffer pH4 to prepare ~0.5mg/ml working stock solution. For higher pH, dilute with relevant buffer to 10µg/ml. The antigen has limited solubility in higher concentrations. Non-sterile product; filter before use in cell culture.
Function
Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Shipping Conditions
Available
Storage Conditions
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Other Gene Names
Sclerostin
Subcellular Location
Secreted, extracellular space, extracellular matrix
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog