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GPHB5 Thyrostimulin Beta Human Recombinant Protein

GPHB5 Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a total molecular mass of 13.34 kDa. The Thyrostimulin contains His tag which consists of 14 additional amino acids. The amino acid sequence of the recombinant human Thyrostimulin beta subunit is 100% homologous to the amino acid sequence of the human Thyrostimulin beta subunit without signal sequence. (N-terminal 24AA) . Thyrostimulin is purified by proprietary chromatographic techniques.

Product Specifications

Synonyms

Glycoprotein hormone beta-5; ZLUT1; GPHB5; GPB5

Gene Name

GPHB5

UniProt

Q86YW7

Reactivity

Human

Tissue Specificity

Highly expressed in brain and at low levels in pituitary. Also found in retina, testis and skin but not in pancreas, parotid, kidney, stomach, liver, colon, small intestine, thyroid, brain or adrenal gland. In pituitary, colocalizes with ACTH, suggesting that it is located in corticotrophs.

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Form

Filtered White lyophilized (freeze-dried) powder.

Reconstitution

Add 0.1M acetate buffer pH4 to prepare ~0.5mg/ml working stock solution. For higher pH, dilute with relevant buffer to 10µg/ml. GPHB5 has limited solubility in higher concentrations. Non-sterile product; filter before use in cell culture.

Function

Stimulates the thyroid. Binds and activates THSR, leading to increased cAMP production.

Shipping Conditions

Available

Storage Conditions

Lyophilized Thyrostimulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, GPHB5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PROTQ86YW7

Product MSDS

https://www.bosterbio.com/msds?sku=PROTQ86YW7

Other Gene Names

Glycoprotein hormone beta-5

Subcellular Location

Secreted.

Available Sizes

Curated Selection

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