VEGF-E Vascular Endothelial Growth Factor-E Recombinant Protein (Orf Virus)

A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.

Product Specifications

Synonyms

VEGF

Gene Name

VEGF

UniProt

Q2F842

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 90.0% as determined by SDS-PAGE.

Bioactivity

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

Shipping Conditions

Available

Storage Conditions

Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.