PSAP Prosaposin Human Recombinant Protein
PSAP Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 17-524) containing a total of 518 amino acids, having a molecular mass of 57.7kDa (calculated), though it migrates at approximately 65kDa on SDS PAGE, the PSAP is fused to a 2 a.a N-terminal linker, a 2 a.a C-terminal linker and a 6 a.a His tag at C-Terminus. The Human PSAP is purified by proprietary chromatographic techniques.
Product Specifications
Synonyms
Prosaposin; Proactivator polypeptide; PSAP; GLBA; SAP1
Gene Name
PSAP
UniProt
P07602
Reactivity
Human
Source
HEK293
Sequence
[object Object]
Purification
Greater than 95.0% as determined by SDS-PAGE.
Form
Filtered White lyophilized (freeze-dried) powder.
Reconstitution
Prepare about 0.5mg/ml working stock solution with deionized water and let the lyophilized pellet dissolve completely. PSAP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Function
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46) . Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.
Shipping Conditions
Available
Storage Conditions
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Other Gene Names
Prosaposin
Subcellular Location
Lysosome
Available Sizes
Frequently Asked Questions
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