Complement Component C5a Mouse Recombinant Protein

C5a Mouse Recombinant produced in E. coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.

Product Specifications

Synonyms

Complement C5; Hemolytic complement; C5; Hc; He; C5a

Gene Name

UniProt

P06684

Reactivity

Mouse

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Bioactivity

The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Function

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

Shipping Conditions

Available

Storage Conditions

Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.