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VEGF-C Vascular Endothelial Growth Factor-C (152 a.a) Rat Recombinant Protein

Selected Variant

PROTO35757-01

Size: 2 µg

Vascular Endothelial Growth Factor -C 152 Rat Recombinant contains 152 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

Product Specifications

Synonyms

VEGF-C152; Vascular endothelial growth factor C 152; VRP; Flt4 ligand; Flt4-L

Gene Name

VEGFC

UniProt

O35757

Reactivity

Rat

Tissue Specificity

Highly expressed in the lung, ovary, preputial gland and the adrenal gland. Expressed in the post-pubertal mammary glands.

Source

Sf9 insect cells

Purification

Greater than 90.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Bioactivity

Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT -4 cells) . The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6KUnits/mg.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Function

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.

Shipping Conditions

Available

Storage Conditions

Lyophilized Vascular Endothelial Growth Factor-C152 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C 152 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Other Gene Names

Vascular endothelial growth factor C

Subcellular Location

Secreted.

Available Sizes

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