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Human ANGPTL7 PicoKine® Quick ELISA Kit, Carrier-free

Selected Variant

FEK2035-carrier-free

Size: 96 Well With Removable Strips

Human ANGPTL7 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45?50 kDa glycoprotein member of the angiopoietin?like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up?regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up?regulated under conditions of increased ocular pressure.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>140</td><td>129</td><td>118</td><td>129</td><td>129</td><td>7.77</td><td>6.0%</td></tr><tr><td>Sample 2</td><td>644</td><td>660</td><td>702</td><td>583</td><td>647</td><td>42.71</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>1907</td><td>2152</td><td>2078</td><td>2215</td><td>2088</td><td>115.20</td><td>5.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356

Gene Name

angiopoietin like 7

Gene ID

10218

UniProt

O43827

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q27-P346

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human ANGPTL7

Anticoagulant

heparin or EDTA

Protein Name

Angiopoietin-related protein 7

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