Human ANGPTL2 PicoKine® Quick ELISA Kit, Carrier-free

Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>590</td><td>572</td><td>610</td><td>690</td><td>615</td><td>45.06</td><td>7.3%</td></tr><tr><td>Sample 2</td><td>1670</td><td>1703</td><td>1941</td><td>1771</td><td>1771</td><td>104.55</td><td>5.9%</td></tr><tr><td>Sample 3</td><td>7933</td><td>8323</td><td>9098</td><td>7778</td><td>8283</td><td>510.72</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4