Rat IGF-2 PicoKine® Quick ELISA Kit, Carrier-free

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>144</td><td>161</td><td>158</td><td>144</td><td>151</td><td>7.82</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>684</td><td>710</td><td>817</td><td>695</td><td>726</td><td>53.05</td><td>7.3%</td></tr><tr><td>Sample 3</td><td>1763</td><td>1688</td><td>1647</td><td>1661</td><td>1689</td><td>44.78</td><td>2.6%</td></tr></table>*number of samples for each test n=16.

Insulin-like growth factor II;IGF-II;Multiplication-stimulating activity;MSA;Multiplication-stimulating polypeptide;Insulin-like growth factor II;Preptin;Igf2;Igf-2;

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)