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Human CCL18/PARC PicoKine® Quick ELISA Kit, Carrier-free

Selected Variant

FEK0686-carrier-free

Size: 96 Well With Removable Strips

Human CCL18/PARC PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human CCL18 in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>18</td><td>17</td><td>18</td><td>18</td><td>17</td><td>0.43</td><td>2.5%</td></tr><tr><td>Sample 2</td><td>49</td><td>56</td><td>49</td><td>48</td><td>50</td><td>3.2</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>269</td><td>295</td><td>278</td><td>296</td><td>284</td><td>11.45</td><td>4%</td></tr></table>*number of samples for each test n=16.

Gene Name

CCL18

Gene ID

6362

UniProt

P55774

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-A89

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<2 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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