Human/Canine/Porcine Insulin ELISA Kit PicoKine®, Carrier-free

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pmol/L)</th><th>Lot 2 (pmol/L)</th><th>Lot 3 (pmol/L)</th><th>Lot 4 (pmol/L)</th><th>Mean (pmol/L)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>28</td><td>18</td><td>24</td><td>19</td><td>22</td><td>1.69</td><td>7.6%</td></tr><tr><td>Sample 2</td><td>93</td><td>93</td><td>112</td><td>85</td><td>96</td><td>6.96</td><td>7.3%</td></tr><tr><td>Sample 3</td><td>474</td><td>411</td><td>299</td><td>444</td><td>407</td><td>21.08</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)