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Mouse ECM1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2284-carrier-free

Size: 96 Well With Removable Strips

Mouse ECM1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ecm1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Extracellular matrix protein 1 is a protein that in humans is encoded by the ECM1 gene. This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>287</td><td>225</td><td>217</td><td>307</td><td>259</td><td>14.22</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>1206</td><td>942</td><td>1057</td><td>968</td><td>1043</td><td>33.18</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>3645</td><td>3059</td><td>2552</td><td>4810</td><td>3517</td><td>158.24</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

extracellular matrix protein 1

UniProt

Q61508

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-E559

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<20 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

63 kDa

Anticoagulant

heparin or EDTA

Protein Name

Extracellular matrix protein 1

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