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Mouse MARCO ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2263-carrier-free

Size: 96 Well With Removable Strips

Mouse MARCO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MARCO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the <a href="https://www.bosterbio.com/anti-macrophage-receptor-marco-marco-antibody-a00391-boster.html" style="color:#EA8D28">MARCO gene</a>. The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>555</td><td>600</td><td>343</td><td>464</td><td>491</td><td>21.29</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>709</td><td>1133</td><td>780</td><td>1104</td><td>932</td><td>47.79</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>7761</td><td>5570</td><td>7930</td><td>9513</td><td>7694</td><td>168.49</td><td>2.2%</td></tr></table>*number of samples for each test n=16.

Gene Name

Marco

Gene ID

17167

UniProt

Q60754

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q70-S518

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<150 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Marco

Anticoagulant

heparin or EDTA

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