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Human PCPE1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2262-carrier-free

Size: 96 Well With Removable Strips

Human PCPE1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PCPE1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>952</td><td>1166</td><td>1130</td><td>693</td><td>985</td><td>50.15</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>1335</td><td>2149</td><td>1773</td><td>1456</td><td>1668</td><td>39.37</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>18623</td><td>16083</td><td>10251</td><td>14876</td><td>14868</td><td>793.96</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Gene Name

PCOLCE

Gene ID

5118

UniProt

Q15113

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-D449

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<300 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PCOLCE

Anticoagulant

heparin or EDTA

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