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Human NAGA ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2248-carrier-free

Size: 96 Well With Removable Strips

Human NAGA PicoKine® ELISA Kit (96 Tests). Quantitate Human NAGA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>23</td><td>20</td><td>26</td><td>29</td><td>25</td><td>1.04</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>49</td><td>56</td><td>33</td><td>53</td><td>48</td><td>3.18</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>424</td><td>272</td><td>483</td><td>282</td><td>365</td><td>28.75</td><td>7.9%</td></tr></table>*number of samples for each test n=16.

Gene Name

NAGA

Gene ID

4668

UniProt

P17050

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L18-Q411

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<8 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NAGA

Anticoagulant

heparin or EDTA

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