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Human ASAHL ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2246-carrier-free

Size: 96 Well With Removable Strips

Human ASAHL PicoKine®® Quick ELISA Kit PicoKine® ELISA Kit (96 Tests). Quantitate Human ASAHL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

N-acylethanolamine-hydrolyzing acid amidase is an enzyme that in humans is encoded by the NAAA gene. Enables N-(long-chain-acyl)ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>30</td><td>30</td><td>24</td><td>25</td><td>27</td><td>1.71</td><td>6.3%</td></tr><tr><td>Sample 2</td><td>48</td><td>36</td><td>49</td><td>59</td><td>48</td><td>2.75</td><td>5.7%</td></tr><tr><td>Sample 3</td><td>437</td><td>401</td><td>423</td><td>452</td><td>428</td><td>22.18</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Gene Name

NAAA

Gene ID

27163

UniProt

Q02083

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: M1-K359

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<8 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NAAA

Anticoagulant

heparin, EDTA or citrate

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