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Human Layilin ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2244-carrier-free

Size: 96 Well With Removable Strips

Human Layilin PicoKine® ELISA Kit (96 Tests). Quantitate Human Layilin in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Layilin is a protein that in humans is encoded by the LAYN gene. Layilin is a type I transmembrane protein that acts as a receptor for hyaluronan. It has an extracellular C-type lectin domain and a 123 aa cytoplasmic domain that interacts with the cytoskeleton. The amino acid sequence of recombinant human Layilin is 85%, 82%, 80%, 79% and 76% identical to that of hamster, canine, bovine, mouse and rat.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>101</td><td>76</td><td>96</td><td>84</td><td>89</td><td>5.39</td><td>6%</td></tr><tr><td>Sample 2</td><td>203</td><td>223</td><td>242</td><td>159</td><td>207</td><td>5.75</td><td>2.8%</td></tr><tr><td>Sample 3</td><td>1553</td><td>1311</td><td>1828</td><td>1209</td><td>1475</td><td>42.04</td><td>2.9%</td></tr></table>*number of samples for each test n=16.

Gene Name

LAYN

Gene ID

143903

UniProt

Q6UX15

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-E220

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<30 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LAYN

Anticoagulant

heparin or EDTA

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