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Human LPL ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2238-carrier-free

Size: 96 Well With Removable Strips

Human LPL PicoKine® ELISA Kit (96 Tests). Quantitate Human LPL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 40 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>137</td><td>100</td><td>85</td><td>132</td><td>114</td><td>3.71</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>201</td><td>296</td><td>166</td><td>236</td><td>225</td><td>4.72</td><td>2.1%</td></tr><tr><td>Sample 3</td><td>2403</td><td>2329</td><td>2427</td><td>2205</td><td>2341</td><td>163.17</td><td>7%</td></tr></table>*number of samples for each test n=16.

Gene Name

LPL

Gene ID

4023

UniProt

P06858

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: A28-G475

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<40 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LPL

Anticoagulant

heparin or EDTA

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