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Human CD49A ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2225-carrier-free

Size: 96 Well With Removable Strips

Human CD49A PicoKine® ELISA Kit (96 Tests). Quantitate Human CD49A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>161</td><td>268</td><td>173</td><td>196</td><td>200</td><td>5.83</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>592</td><td>625</td><td>368</td><td>345</td><td>483</td><td>19.35</td><td>4%</td></tr><tr><td>Sample 3</td><td>3907</td><td>3743</td><td>3150</td><td>4519</td><td>3830</td><td>288.76</td><td>7.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

ITGA1

Gene ID

3672

UniProt

P56199

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: M1-P1141

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<75 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ITGA1

Anticoagulant

heparin or EDTA

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