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Human HS6ST1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2220-carrier-free

Size: 96 Well With Removable Strips

Human HS6ST1 PicoKine® ELISA Kit (96 Tests). Quantitate Human HS6ST1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 7 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>22</td><td>21</td><td>18</td><td>24</td><td>21</td><td>0.7</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>189</td><td>174</td><td>163</td><td>178</td><td>176</td><td>7.92</td><td>4.5%</td></tr><tr><td>Sample 3</td><td>477</td><td>455</td><td>436</td><td>492</td><td>465</td><td>27.44</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Gene Name

HS6ST1

Gene ID

9394

UniProt

O60243

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: P28-W401

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<7 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human HS6ST1

Anticoagulant

heparin or EDTA

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