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Human B7-H2/ICOSL ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2217-carrier-free

Size: 96 Well With Removable Strips

Human B7-H2/ICOSL PicoKine® ELISA Kit (96 Tests). Quantitate Human B7-H2/ICOSL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>2015</td><td>2162</td><td>1984</td><td>1947</td><td>2027</td><td>99.32</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>4613</td><td>4696</td><td>4536</td><td>4799</td><td>4661</td><td>270.34</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>23647</td><td>25479</td><td>24519</td><td>22796</td><td>24020</td><td>1393.17</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Gene Name

ICOSLG

Gene ID

23308

UniProt

O75144

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S258

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<300 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ICOSLG

Anticoagulant

heparin or EDTA

Price

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