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Human CPB1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2180-carrier-free

Size: 96 Well With Removable Strips

Human CPB1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CPB1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>546</td><td>565</td><td>542</td><td>551</td><td>551</td><td>23.69</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>1734</td><td>1726</td><td>1754</td><td>1771</td><td>1746</td><td>80.33</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>7234</td><td>7302</td><td>7165</td><td>7139</td><td>7210</td><td>410.97</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Gene Name

CPB1

Gene ID

1360

UniProt

P15086

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H16-Y417

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human CPB1

Price

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