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Human CFHR1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2165-carrier-free

Size: 96 Well With Removable Strips

Human CFHR1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CFHR1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement factor H-related protein 1 is a protein that in humans is encoded by the CFHR1 gene. This gene encodes a secreted protein belonging to the complement factor H protein family. It binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic-uremic syndrome.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>754</td><td>756</td><td>781</td><td>762</td><td>763</td><td>22.13</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>1625</td><td>1702</td><td>1698</td><td>1675</td><td>1675</td><td>43.55</td><td>2.6%</td></tr><tr><td>Sample 3</td><td>10264</td><td>10126</td><td>10365</td><td>10612</td><td>10342</td><td>341.28</td><td>3.3%</td></tr></table>*number of samples for each test n=16.

Gene Name

CFHR1

Gene ID

3078

UniProt

Q03591

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-R330

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<20 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CFHR1

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