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Mouse CD99L2 ELISA Kit PicoKine®, Carrier-free

Mouse CD99L2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd99l2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to X; X A7.3. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>467</td><td>427</td><td>448</td><td>472</td><td>453</td><td>17.72</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>1310</td><td>1396</td><td>1545</td><td>1357</td><td>1402</td><td>87.99</td><td>6.2%</td></tr><tr><td>Sample 3</td><td>7832</td><td>7176</td><td>7216</td><td>8274</td><td>7624</td><td>456.32</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

CD99 antigen-like protein 2; MIC2-like protein 1; CD99; Cd99l2; Mic2l1

Gene Name

CD99 antigen-like 2

Gene ID

171486

UniProt

Q8BIF0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-G161

Tissue Specificity

Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils. Almost undetectable in bone marrow-derived neutrophils. Also expressed in thymocytes with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver; however, expression in heart, kidney and liver seems restricted to endothelial cells. Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells. Low expression in spleen.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CD99L2

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.

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