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Human CD99L2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2090-carrier-free

Size: 96 Well With Removable Strips

Human CD99L2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD99L2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to Xq28. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>364</td><td>349</td><td>378</td><td>353</td><td>361</td><td>11.24</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>2379</td><td>2185</td><td>2390</td><td>2124</td><td>2269</td><td>117.06</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>7370</td><td>7573</td><td>8346</td><td>7810</td><td>7774</td><td>364.72</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

CD99 antigen-like protein 2; MIC2-like protein 1; CD99; CD99L2; MIC2L1; UNQ1964; PRO4486

Gene Name

CD99 molecule like 2

Gene ID

83692

UniProt

Q8TCZ2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-G185

Tissue Specificity

Expressed in many tissues, with low expression in thymus.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD99L2

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.

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