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Mouse CREG1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1950-carrier-free

Size: 96 Well With Removable Strips

Mouse CREG1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Creg1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Protein CREG1 (Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. It is mapped to 1; 1 H2.3. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>262</td><td>223</td><td>221</td><td>252</td><td>239</td><td>17.86</td><td>7.4%</td></tr><tr><td>Sample 2</td><td>677</td><td>673</td><td>789</td><td>683</td><td>705</td><td>48.33</td><td>6.8%</td></tr><tr><td>Sample 3</td><td>4031</td><td>3933</td><td>3479</td><td>3497</td><td>3735</td><td>249.49</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Protein CREG1; Cellular repressor of E1A-stimulated genes 1; Creg1; Creg

Gene Name

cellular repressor of E1A-stimulated genes 1

Gene ID

433375

UniProt

O88668

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R32-Q220

Tissue Specificity

Widely expressed.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CREG1

Anticoagulant

heparin

Subcellular Location

Secreted.

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