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Mouse CD300b ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1865-carrier-free

Size: 96 Well With Removable Strips

Mouse CD300b ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd300lb in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD300 molecule like family member b is a protein that in humans is encoded by the CD300LB gene. It is mapped to 11; 11 E2. CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>19</td><td>21</td><td>21</td><td>19</td><td>20</td><td>1.00</td><td>5.0%</td></tr><tr><td>Sample 2</td><td>70</td><td>67</td><td>57</td><td>66</td><td>65</td><td>4.84</td><td>7.4%</td></tr><tr><td>Sample 3</td><td>278</td><td>270</td><td>278</td><td>255</td><td>270</td><td>9.39</td><td>3.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

CMRF35-like molecule 7; CLM-7; CD300 antigen-like family member B; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; CD300b; Cd300lb; Irem3; Lmir5

Gene Name

CD300 molecule like family member B

Gene ID

217304

UniProt

Q3U497

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I18-P209

Tissue Specificity

Expressed in myeloid cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Acts as an activating immune receptor in mast cells through its interaction with ITAM-bearing adapter TYROBP.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CD300b

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

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