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Rat EphA4 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1800-carrier-free

Size: 96 Well With Removable Strips

Rat EphA4 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Epha4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 9q33. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>47</td><td>55</td><td>52</td><td>55</td><td>52</td><td>3.26</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>150</td><td>132</td><td>148</td><td>145</td><td>143</td><td>7.01</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>534</td><td>536</td><td>553</td><td>568</td><td>547</td><td>13.82</td><td>2.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Eph receptor A4; Epha4

Gene Name

Eph receptor A4

Gene ID

316539

UniProt

D3ZZK3

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-T547

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat EphA4

Anticoagulant

heparin or EDTA

Subcellular Location

Endoplasmic reticulum. Early endosome membrane. Golgi apparatus. Mitochondrial outer membrane.

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