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Mouse Contactin-4 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1781-carrier-free

Size: 96 Well With Removable Strips

Mouse Contactin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cntn4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>451</td><td>511</td><td>512</td><td>479</td><td>488</td><td>25.27</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>1849</td><td>2120</td><td>1840</td><td>2095</td><td>1976</td><td>131.83</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>7615</td><td>7780</td><td>8028</td><td>8212</td><td>7908</td><td>228.60</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; Cntn4; Kiaa3024

Gene Name

contactin 4

Gene ID

269784

UniProt

Q69Z26

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S1000

Tissue Specificity

Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Contactin-4

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted. Cell membrane. GPI-anchor.

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