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Rat DNER ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2082-carrier-free

Size: 96 Well With Removable Strips

Rat DNER ELISA Kit PicoKine® (96 Tests). Quantitate Rat Dner in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 9q35. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>374</td><td>333</td><td>332</td><td>377</td><td>354</td><td>21.52</td><td>6.0%</td></tr><tr><td>Sample 2</td><td>1696</td><td>1582</td><td>1441</td><td>1656</td><td>1593</td><td>97.21</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>6130</td><td>6281</td><td>6503</td><td>6976</td><td>6472</td><td>319.53</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Delta/notch-like EGF repeat containing; Dner

Gene Name

delta/notch-like EGF repeat containing

Gene ID

316573

UniProt

Q5U215

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-Y649

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat DNER

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Integral component of membrane.

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