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Mouse DNER ELISA Kit PicoKine®, Carrier-free

Mouse DNER ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dner in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 1; 1 C5. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>362</td><td>358</td><td>322</td><td>337</td><td>344</td><td>16.20</td><td>4.7%</td></tr><tr><td>Sample 2</td><td>1554</td><td>1714</td><td>1606</td><td>1723</td><td>1649</td><td>71.71</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>8817</td><td>9078</td><td>8422</td><td>9894</td><td>9052</td><td>538.92</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Delta and Notch-like epidermal growth factor-related receptor; Brain EGF repeat-containing transmembrane protein; Dner; Bet; Bret

Gene Name

delta/notch-like EGF repeat containing

Gene ID

227325

UniProt

Q8JZM4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-Y640

Tissue Specificity

Specifically expressed in brain neurons (at protein level).

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Function

Mediates neuron-glia interaction during astrocytogenesis. May promote differentiation of Bergmann glia during cerebellar development by activating DELTEX-dependent NOTCH1 signaling.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse DNER

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

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