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Mouse GFRA3 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2076-carrier-free

Size: 96 Well With Removable Strips

Mouse GFRA3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gfra3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GDNF family receptor alpha-3 (GFRα3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 18; 18 B1. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>26</td><td>27</td><td>26</td><td>25</td><td>26</td><td>0.70</td><td>2.6%</td></tr><tr><td>Sample 2</td><td>109</td><td>109</td><td>105</td><td>93</td><td>104</td><td>6.55</td><td>6.2%</td></tr><tr><td>Sample 3</td><td>355</td><td>365</td><td>388</td><td>351</td><td>364</td><td>14.35</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; Gfra3

Gene Name

glial cell line derived neurotrophic factor family receptor alpha 3

Gene ID

14587

UniProt

O35118

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N29-N371

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse GFRA3

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cell membrane. GPI-anchor.

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