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Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2063-carrier-free

Size: 96 Well With Removable Strips

Mouse Vitamin DBP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse GC in cell culture supernatants, cell lysates, serum, plasma (heparin), saliva and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>551</td><td>505</td><td>496</td><td>595</td><td>536</td><td>39.57</td><td>7.3%</td></tr><tr><td>Sample 2</td><td>2528</td><td>2531</td><td>2745</td><td>2357</td><td>2540</td><td>137.6</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>8207</td><td>9826</td><td>9283</td><td>9589</td><td>9226</td><td>619.14</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor; Gc-MAF; GcMAF; Gc-globulin; Group-specific component; Gc; Vitamin D-binding protein-macrophage activating factor; DBP-maf; GC

Gene Name

GC, vitamin D binding protein

Gene ID

14473

UniProt

P21614

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L17-S476

Tissue Specificity

Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.

Applications

ELISA

Standard

200 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum, plasma (heparin), saliva and urine.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<50 pg/ml

Function

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Vitamin DBP

Anticoagulant

heparin

Subcellular Location

Secreted.

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