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Human CLEC14A ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2051-carrier-free

Size: 96 Well With Removable Strips

Human CLEC14A ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC14A in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. This gene is mapped to 14q21.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>247</td><td>237</td><td>237</td><td>234</td><td>238</td><td>4.91</td><td>2.0%</td></tr><tr><td>Sample 2</td><td>1236</td><td>1184</td><td>1263</td><td>1230</td><td>1228</td><td>28.41</td><td>2.3%</td></tr><tr><td>Sample 3</td><td>4806</td><td>4354</td><td>5047</td><td>4709</td><td>4729</td><td>249.03</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-type lectin domain family 14 member A; Epidermal growth factor receptor 5; EGFR-5; CLEC14A; C14orf27; EGFR5; UNQ236; PRO269

Gene Name

C-type lectin domain containing 14A

Gene ID

161198

UniProt

Q86T13

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-A490

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CLEC14A

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Membrane. Single-pass type I membrane protein.

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