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Human GAS1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK2044-carrier-free

Size: 96 Well With Removable Strips

Human GAS1 ELISA Kit PicoKine® (96 Tests). Quantitate Human GAS1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 9q21.33. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>55</td><td>53</td><td>50</td><td>52</td><td>52</td><td>1.80</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>228</td><td>261</td><td>228</td><td>264</td><td>245</td><td>17.28</td><td>7.0%</td></tr><tr><td>Sample 3</td><td>845</td><td>788</td><td>838</td><td>845</td><td>829</td><td>23.84</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth arrest-specific protein 1; GAS-1; GAS1

Gene Name

growth arrest specific 1

Gene ID

2619

UniProt

P54826

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L40-S318

Applications

ELISA

Standard

2 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GAS1

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane. GPI-anchor.

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