Rat Midkine ELISA Kit PicoKine®, Carrier-free

Midkine (MK) is a member of a family of neurotrophic and developmentally-regulated heparin-binding molecules that currently consists of midkine, pleiotrophin/PTN and avian RI-HB. This gene is mapped to 3q24. Across species, MK shows 87% amino acid identity between the human and mouse proteins. Between family members, human MK is approximately 50% identical to human PTN.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>181</td><td>188</td><td>196</td><td>190</td><td>188</td><td>5.35</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>632</td><td>606</td><td>618</td><td>625</td><td>620</td><td>9.6</td><td>1.5%</td></tr><tr><td>Sample 3</td><td>1287</td><td>1247</td><td>1348</td><td>1315</td><td>1299</td><td>37.09</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)