Human SEMA4G ELISA Kit PicoKine®, Carrier-free

Human SEMA4G ELISA Kit PicoKine® (96 Tests). Quantitate Human SEMA4G in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 10q24.31. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>27</td><td>23</td><td>24</td><td>24</td><td>24</td><td>1.50</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>102</td><td>98</td><td>104</td><td>102</td><td>101</td><td>2.17</td><td>2.1%</td></tr><tr><td>Sample 3</td><td>468</td><td>537</td><td>490</td><td>506</td><td>500</td><td>25.14</td><td>5.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Semaphorin-4G; SEMA4G; KIAA1619

Gene Name

semaphorin 4G

Gene ID

57715

UniProt

Q9NTN9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-L675

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface receptor for PLXNB2. May play a role in axon guidance.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)