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Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®, Carrier-free

Human TCN2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TCN2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>285</td><td>292</td><td>319</td><td>286</td><td>295</td><td>13.82</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>1139</td><td>1247</td><td>1212</td><td>1220</td><td>1204</td><td>39.97</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>5792</td><td>6174</td><td>5259</td><td>6098</td><td>5830</td><td>359.73</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2

Gene Name

transcobalamin 2

Gene ID

6948

UniProt

P20062

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-W427

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TCN2

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Transcobalamin-2

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