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Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine®, Carrier-free

Mouse Osteoadherin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Omd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 13; 13 A5. Osteoadherin is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>279</td><td>280</td><td>286</td><td>293</td><td>284</td><td>5.59</td><td>1.9%</td></tr><tr><td>Sample 2</td><td>1140</td><td>983</td><td>1062</td><td>973</td><td>1039</td><td>67.49</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>4460</td><td>4445</td><td>3951</td><td>4475</td><td>4332</td><td>220.65</td><td>5.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; Omd

Gene Name

osteomodulin

Gene ID

27047

UniProt

O35103

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I423

Tissue Specificity

Bone specific.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

May be implicated in biomineralization processes. Has a function in binding of osteoblasts via the alpha (V)beta3-integrin.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Osteoadherin

Anticoagulant

heparin or EDTA

Subcellular Location

Extracellular matrix.

Protein Name

Osteomodulin

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