Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®, Carrier-free

Human Osteoadherin ELISA Kit PicoKine® (96 Tests). Quantitate Human OMD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20?30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C?terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>178</td><td>162</td><td>182</td><td>159</td><td>170</td><td>9.9</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>606</td><td>547</td><td>628</td><td>536</td><td>579</td><td>38.73</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>1031</td><td>1040</td><td>1142</td><td>1024</td><td>1059</td><td>48.11</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; OMD; SLRR2C; UNQ190; PRO216

Gene Name

osteomodulin

Gene ID

4958

UniProt

Q99983

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-E421

Tissue Specificity

Bone specific.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

May be implicated in biomineralization processes. Has a function in binding of osteoblasts via the alpha (V)beta3-integrin.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)