Human CLEC1B ELISA Kit PicoKine®, Carrier-free

Human CLEC1B ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC1B in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C-type lectin domain family 1 member B is a protein that in humans is encoded by the CLEC1B gene. This gene is mapped to 12p13.31-p13.2. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>104</td><td>106</td><td>107</td><td>111</td><td>107</td><td>2.54</td><td>2.3%</td></tr><tr><td>Sample 2</td><td>378</td><td>373</td><td>365</td><td>379</td><td>373</td><td>5.53</td><td>1.4%</td></tr><tr><td>Sample 3</td><td>1786</td><td>1782</td><td>1704</td><td>1930</td><td>1800</td><td>81.60</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-type lectin domain family 1 member B; C-type lectin-like receptor 2; CLEC-2; CLEC1B; CLEC2; UNQ721; PRO1384

Gene Name

C-type lectin domain family 1 member B

Gene ID

51266

UniProt

Q9P126

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S55-P229

Tissue Specificity

Expressed preferentially in the liver. Also expressed in immune cells of myeloid origin and on the surface of platelets.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<50 pg/ml

Function

C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN. After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2. (Microbial infection) Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (SYK) and initiation of downstream tyrosine phosphorylation events and activation of PLCG2. (Microbial infection) Acts as an attachment factor for Human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)