Human IL-17F/Interleukin-17F ELISA Kit PicoKine®, Carrier-free

Human IL-17F ELISA Kit PicoKine® (96 Tests). Quantitate Human IL17F in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Members of the interleukin-17 family, such as IL17F, are involved in the regulation of normal versus aberrant T-cell responses.. It is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>121</td><td>134</td><td>142</td><td>126</td><td>130</td><td>7.98</td><td>6.1%</td></tr><tr><td>Sample 2</td><td>432</td><td>464</td><td>435</td><td>494</td><td>456</td><td>25.12</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>1856</td><td>1822</td><td>1758</td><td>1980</td><td>1854</td><td>80.80</td><td>4.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-17F; IL-17F; Cytokine ML-1; IL17F

Gene Name

interleukin 17F

Gene ID

112744

UniProt

Q96PD4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R31-Q163

Tissue Specificity

Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin or EDTA)

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover. Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis. Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)