Human Nephrin ELISA Kit PicoKine®, Carrier-free

Human Nephrin ELISA Kit PicoKine® (96 Tests). Quantitate Human NPHS1 in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. It is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>210</td><td>216</td><td>255</td><td>248</td><td>232</td><td>19.52</td><td>8.4%</td></tr><tr><td>Sample 2</td><td>1142</td><td>1344</td><td>1347</td><td>1325</td><td>1289</td><td>85.57</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>4995</td><td>4549</td><td>4974</td><td>5064</td><td>4895</td><td>202.80</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN

Gene Name

NPHS1, nephrin

Gene ID

4868

UniProt

O60500

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-S1055

Tissue Specificity

Specifically expressed in podocytes of kidney glomeruli.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)