Mouse Myostatin/GDF8 ELISA Kit PicoKine®, Carrier-free

Mouse Myostatin/GDF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>51</td><td>55</td><td>46</td><td>51</td><td>50</td><td>3.19</td><td>6.3%</td></tr><tr><td>Sample 2</td><td>227</td><td>216</td><td>255</td><td>226</td><td>231</td><td>14.50</td><td>6.2%</td></tr><tr><td>Sample 3</td><td>971</td><td>973</td><td>959</td><td>989</td><td>973</td><td>10.67</td><td>1.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth/differentiation factor 8; GDF-8; Myostatin; Mstn; Gdf8

Gene Name

myostatin

Gene ID

17700

UniProt

O08689

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D268-S376

Tissue Specificity

Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, tissue, serum and plasma (heparin or EDTA)

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Acts specifically as a negative regulator of skeletal muscle growth.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)