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Human Serpin A12 ELISA Kit PicoKine®, Carrier-free

Human Serpin A12 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA12 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene is mapped to 14q32.13.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>212</td><td>215</td><td>232</td><td>220</td><td>219</td><td>7.62</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>1109</td><td>1215</td><td>1235</td><td>1149</td><td>1177</td><td>50.53</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>5465</td><td>6356</td><td>5987</td><td>5976</td><td>5946</td><td>317.03</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12

Gene Name

serpin family A member 12

Gene ID

145264

UniProt

Q8IW75

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-K414

Tissue Specificity

Expressed in visceral adipose tissues.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Serpin A12

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

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