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Human TREML2 ELISA Kit PicoKine®, Carrier-free

Human TREML2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TREML2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties. This gene is mapped to 6p21.1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>94</td><td>101</td><td>88</td><td>94</td><td>94</td><td>4.60</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>472</td><td>395</td><td>417</td><td>458</td><td>435</td><td>30.90</td><td>7.1%</td></tr><tr><td>Sample 3</td><td>1928</td><td>1838</td><td>1770</td><td>2074</td><td>1902</td><td>113.77</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Trem-like transcript 2 protein; TLT-2; Triggering receptor expressed on myeloid cells-like protein 2; TREML2; C6orf76, TLT2; UNQ6268/PRO20473

Gene Name

triggering receptor expressed on myeloid cells like 2

Gene ID

79865

UniProt

Q5T2D2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G19-S268

Tissue Specificity

Detected in cultured B-cells, T-cell leukemia and monocyte leukemia. Expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<50 pg/ml

Function

Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TREmL2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

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