Human CD300F ELISA Kit PicoKine®, Carrier-free

Human CD300F ELISA Kit PicoKine® (96 Tests). Quantitate Human CD300LF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>60</td><td>53</td><td>52</td><td>60</td><td>56</td><td>3.76</td><td>6.7%</td></tr><tr><td>Sample 2</td><td>272</td><td>252</td><td>233</td><td>247</td><td>251</td><td>13.98</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>844</td><td>800</td><td>822</td><td>904</td><td>842</td><td>38.76</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD300f; CD300LF; CD300F; CLM1; IGSF13; IREM1; NKIR; UNQ3105/PRO1

Gene Name

CD300 molecule like family member f

Gene ID

146722

UniProt

Q8TDQ1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-S156

Tissue Specificity

Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocy

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apo

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)